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Most Cited Articles of Nature Methods

NIH Image to ImageJ: 25 years of image analysis201231.5K
Fiji: an open-source platform for biological-image analysis201227.8K
Fast gapped-read alignment with Bowtie 2201224.7K
QIIME allows analysis of high-throughput community sequencing data201022.9K
jModelTest 2: more models, new heuristics and parallel computing201210K
Mapping and quantifying mammalian transcriptomes by RNA-Seq20089.7K
A method and server for predicting damaging missense mutations20109.2K
UPARSE: highly accurate OTU sequences from microbial amplicon reads20138.1K
HISAT: a fast spliced aligner with low memory requirements20158K
DADA2: High-resolution sample inference from Illumina amplicon data20167.6K
SignalP 4.0: discriminating signal peptides from transmembrane regions20116.9K
SciPy 1.0: fundamental algorithms for scientific computing in Python20206.2K
Enzymatic assembly of DNA molecules up to several hundred kilobases20095.4K
Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)20065.2K
Universal sample preparation method for proteome analysis20094.7K
Fast and sensitive protein alignment using DIAMOND20154.2K
ModelFinder: fast model selection for accurate phylogenetic estimates20174K
Robust enumeration of cell subsets from tissue expression profiles20153.9K
The I-TASSER Suite: protein structure and function prediction20153.4K
MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy20173.3K
Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position20133.3K
The Perseus computational platform for comprehensive analysis of (prote)omics data20163.3K
Salmon provides fast and bias-aware quantification of transcript expression20173.2K
Deep tissue two-photon microscopy20052.9K
Quantum dots versus organic dyes as fluorescent labels20082.9K