| # | Title | Journal | Year | Citations |
|---|
| 1 | Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors | Gene | 1985 | 16,607 |
| 2 | Site-directed mutagenesis by overlap extension using the polymerase chain reaction | Gene | 1989 | 7,939 |
| 3 | The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers | Gene | 1982 | 6,753 |
| 4 | Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase | Gene | 1988 | 6,310 |
| 5 | Construction and characterization of new cloning vehicle. II. A multipurpose cloning system | Gene | 1977 | 5,154 |
| 6 | Efficient selection for high-expression transfectants with a novel eukaryotic vector | Gene | 1991 | 4,915 |
| 7 | A simple and very efficient method for generating cDNA libraries | Gene | 1983 | 4,906 |
| 8 | Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing | Gene | 1984 | 4,616 |
| 9 | CLUSTAL: a package for performing multiple sequence alignment on a microcomputer | Gene | 1988 | 3,454 |
| 10 | A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments | Gene | 1982 | 3,300 |
| 11 | Construction and characterization of new cloning vehicles. II. A multipurpose cloning system | Gene | 1977 | 3,204 |
| 12 | Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes | Gene | 1995 | 3,112 |
| 13 | Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension | Gene | 1989 | 2,982 |
| 14 | New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites | Gene | 1988 | 2,923 |
| 15 | FACS-optimized mutants of the green fluorescent protein (GFP) | Gene | 1996 | 2,830 |
| 16 | Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis | Gene | 1983 | 2,690 |
| 17 | The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions | Gene | 1988 | 2,616 |
| 18 | A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformaion of Escherichia coli | Gene | 1987 | 2,611 |
| 19 | Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum | Gene | 1994 | 2,547 |
| 20 | Primary structure of the Aequorea victoria green-fluorescent protein | Gene | 1992 | 1,979 |
| 21 | The ‘effective number of codons’ used in a gene | Gene | 1990 | 1,926 |
| 22 | High efficiency transformation of Escherichia coli with plasmids | Gene | 1990 | 1,859 |
| 23 | Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds | Gene | 1995 | 1,802 |
| 24 | A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants | Gene | 1998 | 1,792 |
| 25 | In vitro insertional mutagenesis with a selectable DNA fragment | Gene | 1984 | 1,775 |
| 26 | Epidermal growth factor receptor (EGFR) signaling in cancer | Gene | 2006 | 1,744 |
| 27 | Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant | Gene | 1995 | 1,694 |
| 28 | Multifunctional yeast high-copy-number shuttle vectors | Gene | 1992 | 1,658 |
| 29 | Improved single and multicopy lac-based cloning vectors for protein and operon fusions | Gene | 1987 | 1,622 |
| 30 | Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans | Gene | 2001 | 1,605 |
| 31 | Vectors for selective expression of cloned DNAs by T7 RNA polymerase | Gene | 1987 | 1,594 |
| 32 | Improved broad-host-range plasmids for DNA cloning in Gram-negative bacteria | Gene | 1988 | 1,554 |
| 33 | Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells | Gene | 1979 | 1,537 |
| 34 | Acetylation and deacetylation of non-histone proteins | Gene | 2005 | 1,469 |
| 35 | Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. | Gene | 1992 | 1,301 |
| 36 | A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector | Gene | 1987 | 1,273 |
| 37 | Initiation of translation in prokaryotes and eukaryotes | Gene | 1999 | 1,264 |
| 38 | Genetics and biology of vitamin D receptor polymorphisms | Gene | 2004 | 1,249 |
| 39 | Transformation in yeast: Development of a hybrid cloning vector and isolation of the can1 gene | Gene | 1979 | 1,209 |
| 40 | Specific-purpose plasmid cloning vectors II. Broad host range, high copy number, RSF 1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas | Gene | 1981 | 1,187 |
| 41 | The essence of SNPs | Gene | 1999 | 1,158 |
| 42 | Plasmid screening at high colony density | Gene | 1980 | 1,132 |
| 43 | Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli | Gene | 1991 | 1,102 |
| 44 | Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector | Gene | 1986 | 1,094 |
| 45 | Sterile host yeasts (SHY): A eukaryotic system of biological containment for recombinant DNA experiments | Gene | 1979 | 1,086 |
| 46 | A simple phase-extraction assay for chloramphenicol acyltransferase activity | Gene | 1988 | 1,069 |
| 47 | Construction and characterization of new cloning vehicles I. Ampicillin-resistant derivatives of the plasmid pMB9 | Gene | 1977 | 1,036 |
| 48 | Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli | Gene | 1988 | 1,035 |
| 49 | Thiamine-repressible expression vectors pREP and pRIP for fission yeast | Gene | 1993 | 1,035 |
| 50 | The Sp-family of transcription factors | Gene | 1999 | 1,027 |
