| # | Title | Journal | Year | Citations |
|---|
| 1 | Full-length transcriptome assembly from RNA-Seq data without a reference genome | Nature Biotechnology | 2011 | 17,264 |
| 2 | Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation | Nature Biotechnology | 2010 | 13,805 |
| 3 | Electronics and optoelectronics of two-dimensional transition metal dichalcogenides | Nature Nanotechnology | 2012 | 13,346 |
| 4 | MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification | Nature Biotechnology | 2008 | 12,966 |
| 5 | Single-layer MoS2 transistors | Nature Nanotechnology | 2011 | 12,612 |
| 6 | Integrative genomics viewer | Nature Biotechnology | 2011 | 11,708 |
| 7 | Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2 | Nature Biotechnology | 2019 | 11,167 |
| 8 | Integrating single-cell transcriptomic data across different conditions, technologies, and species | Nature Biotechnology | 2018 | 8,878 |
| 9 | Processable aqueous dispersions of graphene nanosheets | Nature Nanotechnology | 2008 | 8,393 |
| 10 | StringTie enables improved reconstruction of a transcriptome from RNA-seq reads | Nature Biotechnology | 2015 | 8,385 |
| 11 | Second-generation PLINK: rising to the challenge of larger and richer datasets | GigaScience | 2015 | 8,062 |
| 12 | Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences | Nature Biotechnology | 2013 | 8,049 |
| 13 | Model Evaluation Guidelines for Systematic Quantification of Accuracy in Watershed Simulations | Transactions of the ASABE | 2007 | 7,902 |
| 14 | Nanocarriers as an emerging platform for cancer therapy | Nature Nanotechnology | 2007 | 7,469 |
| 15 | Near-optimal probabilistic RNA-seq quantification | Nature Biotechnology | 2016 | 7,322 |
| 16 | Roll-to-roll production of 30-inch graphene films for transparent electrodes | Nature Nanotechnology | 2010 | 7,294 |
| 17 | Black phosphorus field-effect transistors | Nature Nanotechnology | 2014 | 7,071 |
| 18 | Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype | Nature Biotechnology | 2019 | 6,567 |
| 19 | Chemical methods for the production of graphenes | Nature Nanotechnology | 2009 | 6,035 |
| 20 | High-performance lithium battery anodes using silicon nanowires | Nature Nanotechnology | 2008 | 5,860 |
| 21 | Boron nitride substrates for high-quality graphene electronics | Nature Nanotechnology | 2010 | 5,794 |
| 22 | A Real-Time QRS Detection Algorithm | IEEE Transactions on Biomedical Engineering | 1985 | 5,695 |
| 23 | Raman spectroscopy as a versatile tool for studying the properties of graphene | Nature Nanotechnology | 2013 | 5,652 |
| 24 | High-yield production of graphene by liquid-phase exfoliation of graphite | Nature Nanotechnology | 2008 | 5,431 |
| 25 | 3D bioprinting of tissues and organs | Nature Biotechnology | 2014 | 5,158 |
| 26 | 3D Slicer as an image computing platform for the Quantitative Imaging Network | Magnetic Resonance Imaging | 2012 | 5,126 |
| 27 | Intrinsic peroxidase-like activity of ferromagnetic nanoparticles | Nature Nanotechnology | 2007 | 5,080 |
| 28 | Principles of nanoparticle design for overcoming biological barriers to drug delivery | Nature Biotechnology | 2015 | 4,868 |
| 29 | Graphene transistors | Nature Nanotechnology | 2010 | 4,822 |
| 30 | Reviving the lithium metal anode for high-energy batteries | Nature Nanotechnology | 2017 | 4,804 |
| 31 | Quantitative analysis of complex protein mixtures using isotope-coded affinity tags | Nature Biotechnology | 1999 | 4,673 |
| 32 | The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells | Nature Biotechnology | 2014 | 4,652 |
| 33 | Twelve years of SAMtools and BCFtools | GigaScience | 2021 | 4,546 |
| 34 | Large-scale analysis of the yeast proteome by multidimensional protein identification technology | Nature Biotechnology | 2001 | 4,540 |
| 35 | SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler | GigaScience | 2012 | 4,510 |
| 36 | In vivo cancer targeting and imaging with semiconductor quantum dots | Nature Biotechnology | 2004 | 4,460 |
| 37 | Spatial reconstruction of single-cell gene expression data | Nature Biotechnology | 2015 | 4,254 |
| 38 | Ultrasensitive photodetectors based on monolayer MoS2 | Nature Nanotechnology | 2013 | 4,202 |
| 39 | Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein | Nature Biotechnology | 2004 | 4,135 |
| 40 | Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering | Nature Biotechnology | 2005 | 4,068 |
| 41 | Large-area ultrathin films of reduced graphene oxide as a transparent and flexible electronic material | Nature Nanotechnology | 2008 | 4,057 |
| 42 | Molecular Beacons: Probes that Fluoresce upon Hybridization | Nature Biotechnology | 1996 | 3,956 |
| 43 | DNA targeting specificity of RNA-guided Cas9 nucleases | Nature Biotechnology | 2013 | 3,953 |
| 44 | Antimicrobial effects of silver nanoparticles | Nanomedicine: Nanotechnology, Biology, and Medicine | 2007 | 3,939 |
| 45 | Sensitive detection of somatic point mutations in impure and heterogeneous cancer samples | Nature Biotechnology | 2013 | 3,934 |
| 46 | Renal clearance of quantum dots | Nature Biotechnology | 2007 | 3,789 |
| 47 | GREAT improves functional interpretation of cis-regulatory regions | Nature Biotechnology | 2010 | 3,789 |
| 48 | Nano based drug delivery systems: recent developments and future prospects | Journal of Nanobiotechnology | 2018 | 3,689 |
| 49 | Bright light-emitting diodes based on organometal halide perovskite | Nature Nanotechnology | 2014 | 3,627 |
| 50 | Next-generation DNA sequencing | Nature Biotechnology | 2008 | 3,609 |
| 51 | Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes | Nature Biotechnology | 2011 | 3,595 |
| 52 | Nonlinear magic: multiphoton microscopy in the biosciences | Nature Biotechnology | 2003 | 3,524 |
| 53 | Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies | Nature Biotechnology | 2006 | 3,490 |
| 54 | OpenSim: Open-Source Software to Create and Analyze Dynamic Simulations of Movement | IEEE Transactions on Biomedical Engineering | 2007 | 3,477 |
| 55 | Solutions able to reproducein vivo surface-structure changes in bioactive glass-ceramic A-W3 | Journal of Biomedical Materials Research Part B | 1990 | 3,425 |
| 56 | A mechanistic study of the antibacterial effect of silver ions onEscherichia coli andStaphylococcus aureus | Journal of Biomedical Materials Research Part B | 2000 | 3,394 |
| 57 | A clearer vision for in vivo imaging | Nature Biotechnology | 2001 | 3,393 |
| 58 | SignalP 5.0 improves signal peptide predictions using deep neural networks | Nature Biotechnology | 2019 | 3,317 |
| 59 | Control of valley polarization in monolayer MoS2 by optical helicity | Nature Nanotechnology | 2012 | 3,280 |
| 60 | Differential analysis of gene regulation at transcript resolution with RNA-seq | Nature Biotechnology | 2013 | 3,256 |
| 61 | Dimensionality reduction for visualizing single-cell data using UMAP | Nature Biotechnology | 2019 | 3,254 |
| 62 | What is flux balance analysis? | Nature Biotechnology | 2010 | 3,233 |
| 63 | Functionalized graphene sheets for polymer nanocomposites | Nature Nanotechnology | 2008 | 3,206 |
| 64 | Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9 | Nature Biotechnology | 2016 | 3,168 |
| 65 | Optimizing taxonomic classification of marker-gene amplicon sequences with QIIME 2’s q2-feature-classifier plugin | Microbiome | 2018 | 3,159 |
| 66 | Monitoring dopants by Raman scattering in an electrochemically top-gated graphene transistor | Nature Nanotechnology | 2008 | 3,125 |
| 67 | An economic method for the fluorescent labeling of PCR fragments | Nature Biotechnology | 2000 | 3,083 |
| 68 | The Effect of Nanoparticle Size, Shape, and Surface Chemistry on Biological Systems | Annual Review of Biomedical Engineering | 2012 | 3,078 |
| 69 | ISB recommendation on definitions of joint coordinate systems of various joints for the reporting of human joint motion—Part II: shoulder, elbow, wrist and hand | Journal of Biomechanics | 2005 | 3,077 |
| 70 | A Joint Coordinate System for the Clinical Description of Three-Dimensional Motions: Application to the Knee | Journal of Biomechanical Engineering | 1983 | 3,074 |
| 71 | Fabrication of novel biomaterials through molecular self-assembly | Nature Biotechnology | 2003 | 3,048 |
| 72 | Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling | Nature Biotechnology | 2009 | 3,047 |
| 73 | Expression monitoring by hybridization to high-density oligonucleotide arrays | Nature Biotechnology | 1996 | 3,041 |
| 74 | Valley polarization in MoS2 monolayers by optical pumping | Nature Nanotechnology | 2012 | 3,036 |
| 75 | Memristive devices for computing | Nature Nanotechnology | 2013 | 3,019 |
| 76 | Photodetectors based on graphene, other two-dimensional materials and hybrid systems | Nature Nanotechnology | 2014 | 3,017 |
| 77 | Deep Learning in Medical Image Analysis | Annual Review of Biomedical Engineering | 2017 | 2,935 |
| 78 | The impact of the MIT-BIH Arrhythmia Database | IEEE Engineering in Medicine and Biology Magazine | 2001 | 2,903 |
| 79 | Photoplethysmography and its application in clinical physiological measurement | Physiological Measurement | 2007 | 2,898 |
| 80 | Topological properties and dynamics of magnetic skyrmions | Nature Nanotechnology | 2013 | 2,887 |
| 81 | Approaching ballistic transport in suspended graphene | Nature Nanotechnology | 2008 | 2,865 |
| 82 | The use of nanocrystals in biological detection | Nature Biotechnology | 2004 | 2,849 |
| 83 | Skin-like pressure and strain sensors based on transparent elastic films of carbon nanotubes | Nature Nanotechnology | 2011 | 2,839 |
| 84 | A stretchable carbon nanotube strain sensor for human-motion detection | Nature Nanotechnology | 2011 | 2,836 |
| 85 | Sharing and community curation of mass spectrometry data with Global Natural Products Social Molecular Networking | Nature Biotechnology | 2016 | 2,802 |
| 86 | A cross-platform toolkit for mass spectrometry and proteomics | Nature Biotechnology | 2012 | 2,794 |
| 87 | A metal-free bifunctional electrocatalyst for oxygen reduction and oxygen evolution reactions | Nature Nanotechnology | 2015 | 2,782 |
| 88 | High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells | Nature Biotechnology | 2013 | 2,754 |
| 89 | Ultrafast graphene photodetector | Nature Nanotechnology | 2009 | 2,748 |
| 90 | A linear matrix inequality approach to H∞ control | International Journal of Robust and Nonlinear Control | 1994 | 2,742 |
| 91 | Intrinsic and extrinsic performance limits of graphene devices on SiO2 | Nature Nanotechnology | 2008 | 2,730 |
| 92 | Assembly of long, error-prone reads using repeat graphs | Nature Biotechnology | 2019 | 2,730 |
| 93 | What is a support vector machine? | Nature Biotechnology | 2006 | 2,669 |
| 94 | CRISPR-Cas systems for editing, regulating and targeting genomes | Nature Biotechnology | 2014 | 2,648 |
| 95 | Efficient genome editing in zebrafish using a CRISPR-Cas system | Nature Biotechnology | 2013 | 2,638 |
| 96 | PICRUSt2 for prediction of metagenome functions | Nature Biotechnology | 2020 | 2,621 |
| 97 | A standardized bacterial taxonomy based on genome phylogeny substantially revises the tree of life | Nature Biotechnology | 2018 | 2,615 |
| 98 | Metal-halide perovskites for photovoltaic and light-emitting devices | Nature Nanotechnology | 2015 | 2,604 |
| 99 | Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts | Nature Biotechnology | 2008 | 2,583 |
| 100 | Memristive switching mechanism for metal/oxide/metal nanodevices | Nature Nanotechnology | 2008 | 2,578 |
